Articles

Recent Submissions

  • Pehkonen, Henna; Lento, Mira; von Nandelstadh, Pernilla; Filippou, Artemis; Grénman, Reidar; Lehti, Kaisa; Monni, Outi (BioMed Central, 2018)
    Abstract Background PPFIA1 is located at the 11q13 region commonly amplified in cancer. The protein liprin-α1 encoded by PPF1A1 contributes to the adhesive and invasive structures of cytoskeletal elements and is located at the invadosomes in cancer cells. However, the precise mechanism of liprin-α1 function in cancer progression has remained elusive. Methods Invasion regulating activity of liprin-α1 was examined by analyzing the functions of squamous cell carcinoma of head and neck (HNSCC) cell lines in three-dimensional collagen I after RNAi mediated gene knockdown. Transcriptome profiling and Gene Set Enrichment Analysis from HNSCC and breast cancer cells were used to identify expression changes relevant to specific cellular localizations, biological processes and signaling pathways after PPFIA1 knockdown. The significance of the results was assessed by relevant statistical methods (Wald and Benjamini-Hochberg). Localization of proteins associated to liprin-α1 was studied by immunofluorescence in 2D and 3D conditions. The association of PPFIA1 amplification to HNSCC patient survival was explored using The Cancer Genome Atlas data. Results In this study, we show that liprin-α1 regulates biological processes related to membrane microdomains in breast carcinoma, as well as protein trafficking, cell-cell and cell-substrate contacts in HNSCC cell lines cultured in three-dimensional matrix. Importantly, we show that in all these cancer cells liprin-α1 knockdown leads to the upregulation of transmembrane protein CD82, which is a suppressor of metastasis in several solid tumors. Conclusions Our results provide novel information regarding the function of liprin-α1 in biological processes essential in cancer progression. The results reveal liprin-α1 as a novel regulator of CD82, linking liprin-α1 to the cancer cell invasion and metastasis pathways.
  • Rosendahl, Jenni; Fogelholm, Mikael; Pelkonen, Anna; Makela, Mika J.; Makitie, Outi; Erkkola, Maijaliisa (2017)
    Background/Aims: Vitamin D insufficiency is common in children. We aimed to evaluate the main determinants of vitamin D status in Finnish school-aged children, including the history of allergic diseases. Methods: We conducted a cross-sectional study on 171 ten-year-olds where serum 25-hydroxyvitamin D (25[OH] D) levels were measured, and data on food consumption and use of vitamin D supplements were collected. The history of allergic diseases was evaluated with a validated questionnaire. Results: Vitamin D insufficiency (
  • Shin, Young Kyu; Ylä-Anttila, Matti Tuomas (2018)
    The literature on new social risk (NSR) groups, such as single parents and temporary workers, has argued that they are less likely to join trade unions than other employees. It has been suggested that this is due to the unions’ incapacity or unwillingness to promote policies that mediate NSRs. We argue that there are differences in unionization between different NSR groups, and that country-level institutional structures, operationalized here as industrial relations (IR) regimes, have effects on how likely NSR groups are to unionize. Our multilevel logistic models using European Social Survey (ESS) data produce three main results: (1) family policy-related NSR groups (single parents, female employees with children and female caregivers) are more – not less – unionized than the average worker; (2) precarious workers (low-skilled service employees, temporary employees and part-timers) are, indeed, less unionized than average but (3) this result concerns mostly the liberal and transitional IR regimes.
  • Tomašič, Tihomir; Barančoková, Michaela; Zidar, Nace; Ilaš, Janez; Tammela, Päivi Sirpa Marjaana; Kikelj, Danijel (2018)
  • Harris, Vanessa; Ali, Asad; Fuentes, Susana; Korpela, Katri; Kazi, Momin; Tate, Jacqueline; Parashar, Umesh; Wiersinga, W. Joost; Giaquinto, Carlo; de Weerth, Carolina; de Vos, Willem M. (2018)
    Rotavirus (RV) is the leading cause of diarrhea-related death in children worldwide and ninety-five percent of rotavirus deaths occur in Africa and Asia. Rotavirus vaccines (RVV) can dramatically reduce RV deaths, but have low efficacy in low-income settings where they are most needed. The intestinal microbiome may contribute to this decreased RVV efficacy. This pilot study hypothesizes that infants' intestinal microbiota composition correlates with RVV immune responses and that RVV responders have different gut microbiota as compared to non-responders. We conducted a nested, matched case-control study comparing the pre-vaccination intestinal microbiota composition between 10 6-week old Pakistani RVV-responders, 10 6-week old Pakistani RVV non-responders, and 10 healthy Dutch infants. RVV response was defined as an Immunoglobulin A of >= 20 IU/mL following Rotarix (TM)(RV1) vaccination in an infant with aprevaccination IgA RV1 response correlated with a higher relative abundance of bacteria belonging to Clostridium cluster XI and Proteobacteria, including bacteria related to Serratia and Escherichia coli. Remarkably, abundance of these Proteobacteria was also significantly higher in Dutch infants when compared to RV1-non-responders in Pakistan. This small but carefully matched study showed the intestinal microbiota composition to correlate with RV1 seroconversion in Pakistan infants, identifying signatures shared with healthy Dutch infants.
  • Nedoszytko, Boguslaw; Wierzbicki, Piotr; Karenko, Leena; Maciejewska-Radomska, Agata; Stachewicz, Przemyslaw; Zablotna, Monika; Glen, Jolanta; Vakeva, Liisa; Nowicki, Roman J.; Sokolowska-Wojdylo, Malgorzata (2018)
    Introduction: Microbial infection and associated super antigens have been implicated in the pathogenesis of cutaneous T-cell lymphoma (CTCL), and many patients die from complicating bacterial infections. It has been postulated that Chlamydophila pneumoniae (C. pneumoniae) infection may be involved in the pathogenesis of Mycosis fungoides (MF) but published data are limited and controversial. Aim: To analyze the frequency of (C. pneumoniae) DNA presence in blood samples of lymphoma cases. Material and methods: Using Q-PCR method we analyzed the presence of DNA in the blood samples obtained from 57 patients with CTCL (55 - mycosis fungoides (MF)/Sezary syndrome (SS), one primary cutaneous anaplastic large cell lymphoma (CD30+) and one NKT cell lymphoma) and 3 patients with cutaneous B-cell lymphomas, and 120 individuals from control groups (40 patients with psoriasis, 40 patients with atopic dermatitis and 40 healthy controls). Results: Chlamydophila pneumoniae DNA was identified in 13 of 55 cases in the MF/SS group (23.6%), in 1 patient with CD30+ large cell lymphoma and in 1 of 3 patients with B-cell lymphoma. The presence of C. pneumoniae was confirmed in 1 of 40 psoriatic patients (2.5%), in 5 of 40 patients with atopic dermatitis (12.5%) and in none of 40 healthy individuals. Presence of C. pneumoniae DNA in MF patients was strongly associated with disease progression; rs = 0.756; p = 0.0123 for groups IA -> IVB, and was noted more frequently in advanced (III + IV) stages than in early (I-II) stages (p = 0.0139). There are no differences in the mean age of MF/SS patients with and without infection. Conclusions: The presence of C. pneumoniae DNA in the blood cells is a frequent event in late stages of MF/SS and may be explained by Th2 shift and suppression of the immune system during the course of the disease.
  • Delles, Christian; Rankin, Naomi J.; Boachie, Charles; McConnachie, Alex; Ford, Ian; Kangas, Antti; Soininen, Pasi; Trompet, Stella; Mooijaart, Simon P.; Jukema, J. Wouter; Zannad, Faiez; Ala-Korpela, Mika; Salomaa, Veikko; Havulinna, Aki S.; Welsh, Paul; Wurtz, Peter; Sattar, Naveed (2018)
    Aims We investigated the association between quantified metabolite, lipid and lipoprotein measures and incident heart failure hospitalisation (HFH) in the elderly, and examined whether circulating metabolic measures improve HFH prediction.& para;& para;Methods and results Overall, 80 metabolic measures from the PROspective Study of Pravastatin in the Elderly at Risk (PROSPER) trial were measured by proton nuclear magnetic resonance spectroscopy (n = 5341; 182 HFH events during 2.7-year follow-up). We repeated the work in FINRISK 1997 (n = 7330; 133 HFH events during 5-year follow-up). In PROSPER, the circulating concentrations of 13 metabolic measures were found to be significantly different in those who were later hospitalised for heart failure after correction for multiple comparisons. These included creatinine, phenylalanine, glycoprotein acetyls, 3-hydroxybutyrate, and various high-density lipoprotein measures. In Cox models, two metabolites were associated with risk of HFH after adjustment for clinical risk factors and N-terminal pro-B-type natriuretic peptide (NT-proBNP): phenylalanine [hazard ratio (HR) 1.29, 95% confidence interval (CI) 1.10-1.53; P = 0.002] and acetate (HR 0.81, 95% CI 0.68-0.98; P = 0.026). Both were retained in the final model after backward elimination. Compared to a model with established risk factors and NT-proBNP, this model did not improve the C-index but did improve the overall continuous net reclassification index (NRI 0.21; 95% CI 0.06-0.35; P = 0.007) due to improvement in classification of non-cases (NRI 0.14; 95% CI 0.12-0.17; P
  • University of Helsinki, Department of Computer Science; Bacchus, Fahiem; Järvisalo, Matti Juhani; Martins, Ruben; ; ; ; (Department of Computer Science, University of Helsinki, 2018)
    Department of Computer Science Series of Publications B
  • Hagelberg, Nora; Harno, Hanna; Saijonkari, Maija; Isojärvi, Jaana; Mäkelä, Marjukka; Sihvo, Sinikka; Jääskeläinen, Satu (2017)
    Hermoperäisen eli neuropaattisen kivun tai monimuotoisen paikallisen kipuoireyhtymän (CRPS) nykyiset hoito- ja kuntoutusmenetelmät auttavat vain osaa potilaista, joten uusien hoitomuotojen tarve on ilmeinen. Järjestelmälliseen kirjallisuuskatsaukseen haettiin tutkimukset aivojen sarjamagneettistimulaation (rTMS) vaikuttavuudesta ja turvallisuudesta hermovauriokipu- ja CRPS-potilailla, joille muut hoitomenetelmät eivät ole tuoneet riittävää kivun lievitystä. Vaikuttavuusarvio perustuu seitsemään satunnaistettuun, lumekontrolloituun tutkimukseen, joissa oli ¬samanaikainen vertailuryhmä, sekä kolmeen tutkimukseen, joissa potilas sai satunnaistetussa järjestyksessä aktiivista ja lumehoitoa. Useimmissa tutkimuksissa rTMS-hoito lievitti lyhytkestoisesti neuropaattista kipua lumestimulaatioon ¬verrattuna. Kipua lievittävä vaikutus oli suurin heti hoitojakson päättyessä ja pieneni seurannassa. Vaikutus CRPS-kipuun oli merkittävä mutta lyhytkestoinen. Raportoidut haitat olivat lieviä ja vaarattomia. Yleisin haittavaikutus oli päänsärky. Lyhytkestoisten seurantatutkimusten perusteella hermovauriokipupotilaat hyötyvät rTMS-hoidosta, jos muu hoito ei lievitä kipua riittävästi. Menetelmän pitkäaikaisesta vaikuttavuudesta ja ylläpitohoidon malleista tarvitaan lisää tutkimusta.
  • Mäki, Päivi; Lehtinen-Jacks, Susanna; Vuorela, Nina; Levälahti, Esko; Koskela, Timo; Saari, Antti; Mölläri, Kaisa; Mahkonen, Raimo; Salo, Jarmo; Laatikainen, Tiina (2017)
    Lasten ylipainon yleisyydestä on ollut hyvin vähän koko lapsiväestöä edustavaa, mittauksiin perustuvaa tietoa. Tavoitteena oli selvittää, voidaanko tiedonhankinnassa hyödyntää Avohilmo-rekisteriä. Avohilmosta poimittiin tiedot ajalta 1.6.2014–31.5.2015 kaikilta lastenneuvolan ja kouluterveydenhuollon terveydenhoitokäynneillä käyneiltä lapsilta kunnista, joissa pituus- ja painotietojen kattavuus oli vähintään 65 %, eli vain noin viidesosasta kuntia. Ylipainoisia oli noin 25 % 2–16-vuotiaista pojista ja 16 % tytöistä. Pojista 7 % ja tytöistä 3 % oli lihavia. Avohilmo antaa hyvät edellytykset lasten ylipainon yleisyyden seurantaan valtakunnallisesti. Kasvutietojen siirtoa kunnista Avohilmoon tulee kehittää.
  • Hieta, Niina; Hiltunen-Back, Eija (2017)
    •Miehen uretriitin eli virtsaputkitulehduksen oireita ovat vuoto, kirvely ja virtsaputken suun punoitus. •Klamydia- ja tippuritartunnan mahdollisuus tutkitaan kaikilta potilaita ensivirtsan nukleiinihappo-osoitus¬testillä. •Uretriitin voi aiheuttaa myös Mycoplasma genitalium, adenovirus ja herpes simplex -virukset. Emättimen jasuun normaaliflooraan kuuluvat bakteeritkin saattavat olla tulehduksen taustalla. •Kroonisen eturauhastulehduksen mahdollisuus kannattaa ottaa huomioon, jos muut sairaudet on suljettu pois.
  • Helminen, Olli; Knip, Mikael; Veijola, Riitta (2017)
  • Ylikoski, Ari; Partinen, Markku (2017)
    Unettomuuden lisääntyminen ja yöunen rikkonaisuus ovat tavallisia sekä ikääntymiseen että ¬neurodegeneraatioon liittyviä vaivoja. Ihmisen ikääntyessä useat välittäjäaineet vähenevät, kuten myös hermosolut erityisesti sinitumakkeessa ja hypotalamuksen tumakkeissa. Kohorttitutkimuksissa on havaittu, että unihäiriöt edeltävät usein neurodegeneratiivisten sairauksien tyypillisiä ydinoireita. Uuden tutkimustiedon mukaan unihäiriöt voivat altistaa neurodegeneratiivisille sairauksille ja pahentaa niitä. Unihäiriöt kuuluvat näiden sairauksien hoidettavissa oleviin riskitekijöihin. Uni tai sen puute saattavat suojata neurodegeneraatiolta tai altistaa sille usealla eri mekanismilla.
  • Jokelainen, Kalle (2017)
    Pitkittynyt eli yli neljä viikkoa kestänyt ripuli on yleinen vaiva ja yksi tavallisimmista syistä hakeutua lääkärin vastaanotolle. Merkittävä osa potilaista voidaan tutkia ja hoitaa perusterveydenhuollossa. Koska pitkäaikaisen ripulin syynä voi olla joko orgaaninen sairaus tai toiminnallinen vaiva, selvittelyssä on välttämätöntä edetä järjestelmällisesti ja potilaan ikä sekä yksilöllinen oirekuva huomioon ottaen. Mikäli potilaalla esiintyy hälytysoireita, kuten verenvuotoa peräaukosta, painonlaskua tai poikkeava vatsan palpaatiolöydös, tai laboratoriokokeissa ilmenee poikkeavaa, hänet on syytä lähettää gastroenterologin arvioon.